Contact an Account ManagerGentamicin C: Separation of C1 C1a C2 C2a and C2b components by HPLC using isocratic ion-exchange chromatography and postcolumn derivatization
Summary
Type: HPLC
Application #: 103380
Catalog#: 720028.46 - MN HPLC Column, Nucleosil SA, 100A, 10um, 4.6x250mm
Detection: fluorescence, excitation 338nm, emission 455nm
Conditions: eluent: 1.1M KCl solution, adjusted to pH 3.0 with HCl
flow rate 2ml/min
Authors: Seidl G
Source: Chromatographia 25 (1988) 169 - 171
Details
Legend: Fig. 2. Separation of a mixture of 800ng gentamicin and 100ng sagamicin (C2b).